abstract：:The aim of this study was to develop a simple and reliable method to determine the viability of Giardia intestinalis after incubation with an anti-giardial agent by using a colorimetric method. Factors that may affect the optical density value were systematically evaluated. The most suitable conditions were obtained w...

journal_title：Experimental parasitology

authors： Hounkong K,Sawangjaroen N,Phongpaichit S

更新日期：2011-02-01 00:00:00

abstract：:The existence of cholinergic receptors in the immune system cells is well documented. This study aimed to evaluate the acetylcholinesterase activity (AChE) in lymphocytes from rats infected with Trypanosoma evansi in acute and chronic phase disease. Twenty animals were infected with 10(6) trypomastigotes forms each an...

journal_title：Experimental parasitology

authors： Da Silva AS,Monteiro SG,Gonçalves JF,Spanevello R,Schmatz R,Oliveira CB,Costa MM,França RT,Jaques JA,Schetinger MR,Mazzanti CM,Lopes ST

更新日期：2011-02-01 00:00:00

abstract：:The expression and biological role of actin during the Trypanosoma cruzi life cycle remains largely unknown. Polyclonal antibodies against a recombinant T. cruzi actin protein were used to confirm its expression in epimastigotes, trypomastigotes, and amastigotes. Although the overall levels of expression were similar,...

journal_title：Experimental parasitology

authors： Cevallos AM,Segura-Kato YX,Merchant-Larios H,Manning-Cela R,Alberto Hernández-Osorio L,Márquez-Dueñas C,Ambrosio JR,Reynoso-Ducoing O,Hernández R

更新日期：2011-01-01 00:00:00

abstract：:We investigated the effects of the anti-malarials mefloquine and primaquine against the juvenile and adult life stages of Schistosoma mansoniin vitro. Cercariae were incubated with 0.5 μg/ml, 1 μg/ml and 2 μg/ml mefloquine or primaquine and with 1 μg/ml praziquantel for 12h. Schistosomula, pre-adults and adults were i...

journal_title：Experimental parasitology

authors： Holtfreter MC,Loebermann M,Klammt S,Sombetzki M,Bodammer P,Riebold D,Kinzelbach R,Reisinger EC

更新日期：2011-01-01 00:00:00

abstract：:Propylene glycol used as an ophthalmic demulcent in certain contact-lens care systems has been included recently among factors responsible for increasing Acanthamoeba keratitis. In this study, we provide evidence that propylene glycol as well as examined contact-lens solutions containing it induce rapid differentiatio...

journal_title：Experimental parasitology

authors： Kliescikova J,Kulda J,Nohynkova E

更新日期：2011-01-01 00:00:00

abstract：:Shiraz district in south of Iran is a classical focus of Cutaneous leishmaniasis (CL) and previous research has consistently documented the etiologic agent to be Leishmania tropica and Leishmania major in urban and rural areas, respectively. However, none of the Phlebotomus sergenti, a known vector for L. tropica, of ...

journal_title：Experimental parasitology

authors： Oshaghi MA,Rasolian M,Shirzadi MR,Mohtarami F,Doosti S

更新日期：2010-12-01 00:00:00

abstract：:High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were inf...

journal_title：Experimental parasitology

authors： Mehta SR,Zhang XQ,Badaro R,Spina C,Day J,Chang KP,Schooley RT

更新日期：2010-12-01 00:00:00

abstract：:The differential effect of fox and pig bile and its corresponding low molecular weight fraction (LMW) was investigated on the in vitro invasion of MDCK-AA7 epithelial cell monolayers by Trichinella spiralis muscle larvae. Seven invasion experiments were performed and a total of 274 cell monolayers were examined. Fox a...

journal_title：Experimental parasitology

authors： Theodoropoulos G,Petrakos G

更新日期：2010-12-01 00:00:00

abstract：:The protozoan parasite Trypanosoma cruzi, the etiologic agent of Chagas disease, is an obligate intracellular protozoan pathogen. Overlapping mechanisms ensure successful infection, yet the relationship between these cellular events and clinical disease remains obscure. This review explores the process of cell invasio...

journal_title：Experimental parasitology

authors： Epting CL,Coates BM,Engman DM

更新日期：2010-11-01 00:00:00

abstract：:Giardia lamblia is a common cause of both acute and chronic diarrheal disease in humans worldwide. It has been shown that mast cells, IL-6 and TNF-alpha are substantially involved in the early control of G. lamblia infection in mice. However, no studies have yet been reported concerning the interaction between mast ce...

journal_title：Experimental parasitology

authors： Muñoz-Cruz S,Gómez-García A,Millán-Ibarra J,Giono-Cerezo S,Yépez-Mulia L

更新日期：2010-11-01 00:00:00

abstract：:In this first report about pinworms peptidases we primarily characterize peptidases released during in vitro maintenance of two common pinworms of laboratory animals -Syphacia muris and Passalurus ambiguus. The peptidase activity obtained using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showe...

journal_title：Experimental parasitology

authors： Vadlejch J,Lytvynets A,Jankovská I,Langrová I

更新日期：2010-10-01 00:00:00

abstract：:Chlorination is a well-known disinfection method, used in water treatment to inactivate various microorganisms, it induces numerous cellular changes. Even though Acanthamoebae are frequently found in water, the cellular changes induced in Acanthamoebae have not been described in the literature. Acanthamoebae are patho...

journal_title：Experimental parasitology

authors： Mogoa E,Bodet C,Legube B,Héchard Y

更新日期：2010-09-01 00:00:00

abstract：:Little is known about the prevalence of Balamuthia mandrillaris amoebae and Balamuthia amoebic encephalitis in Africa. As an approach, relative concentrations of amoebae-binding serum antibodies (Ab) were assessed by flow cytometry using formaldehyde-fixed B. mandrillaris, Acanthamoeba lenticulata 72-2 and Acanthamoeb...

journal_title：Experimental parasitology

authors： Kiderlen AF,Radam E,Schuster FL,Adjogoua EV,Akoua-Koffi C,Leendertz FH

更新日期：2010-09-01 00:00:00

abstract：:Acanthamoeba are free-living amoebae found in most environments that can cause brain and corneal infections. To infect humans, these pathogens must interact with host cells and the extracellular matrix (ECM). In order to define the mode by which amoebae recognize ECM components and process this recognition, we analyze...

journal_title：Experimental parasitology

authors： Rocha-Azevedo Bd,Jamerson M,Cabral GA,Marciano-Cabral F

更新日期：2010-09-01 00:00:00

abstract：:Three different cell lines (murine macrophages, HeLa and osteosarcoma cells) were assayed in order to check for the manifestation of the cytopathic effects of three strains of Acanthamoeba recently isolated in our laboratory from contact lens cases: CLC-16, CLC-41.r and CLC-51-l. Adhesion and cytotoxicity assays were ...

journal_title：Experimental parasitology

authors： Martín-Navarro CM,Lorenzo-Morales J,Machín RP,López-Arencibia A,Valladares B,Piñero JE

更新日期：2010-09-01 00:00:00

abstract：:A survey was carried out in the metropolitan area of Mexico City to determine the presence of Acanthamoeba in the tap water of houses of contact lens wearers. Water samples were taken from the mains water entry, bathroom sinks and storage containers (roof tanks, cisterns) of 27 houses; and from the solution contained ...

journal_title：Experimental parasitology

authors： Bonilla-Lemus P,Ramírez-Bautista GA,Zamora-Muñoz C,Ibarra-Montes Mdel R,Ramírez-Flores E,Hernández-Martínez MD

更新日期：2010-09-01 00:00:00

abstract：:Trophozoites and cysts of free-living Acanthamoeba castellanii present a serious risk to human health as causative agents of human diseases such as fatal granulomatous amoebic encephalitis and Acanthamoeba keratitis that is reported from various part of the world, also in Poland, with increasing frequency, particularl...

journal_title：Experimental parasitology

authors： Chomicz L,Padzik M,Graczyk Z,Starosciak B,Graczyk TK,Naprawska A,Oledzka G,Szostakowska B

更新日期：2010-09-01 00:00:00

abstract：:In Trypanosoma brucei the GPI-anchored variant surface glycoprotein (VSG) represents approximately 90% of cell surface protein and a major proportion of endoplasmic reticulum (ER) biosynthetic output. We identified four trypanosomatid-specific genes encoding candidate ER-resident proteins; all were required for normal...

journal_title：Experimental parasitology

authors： Wang YN,Wang M,Field MC

更新日期：2010-07-01 00:00:00

abstract：:In the present study, we examined the effect of amphotericin B on larval stages (miracidia and primary sporocyst) of the helminth Schistosoma mansoni, the causative agent of human schistosomiasis. Amphotericin B (AmB) is a polyene macrolide that disturbs the function of the cell membrane; it is widely used as prophyla...

journal_title：Experimental parasitology

authors： Moné Y,Mitta G,Duval D,Gourbal BE

更新日期：2010-06-01 00:00:00

abstract：:Branchial bopyrids infesting porcellanid crabs from the Philippines were investigated based on intertidal collections made in 1999-2000. Crabs of the genus Petrolisthes collected from sites in the northern Philippines were examined and two parasite species were found. One new pseudionine species found infesting Petrol...

journal_title：Experimental parasitology

authors： Williams JD,Madad AZ

更新日期：2010-05-01 00:00:00

abstract：:Larvae of Rhinoestrus spp. (Diptera, Oestridae) infect nasal and sinus cavities of horses, causing a nasal myiasis characterized by severe respiratory distress. Presently, the diagnosis of horse nasal botfly relies on the observation of clinical signs, on the post mortem retrieval of larvae or on molecular assays perf...

journal_title：Experimental parasitology

authors： Milillo P,Traversa D,Elia G,Otranto D

更新日期：2010-04-01 00:00:00

abstract：:Ticks deposit saliva at the site of their attachment to a host in order to inhibit haemostasis, inflammation and innate and adaptive immune responses. The anti-haemostatic properties of tick saliva have been described by many studies, but few show that tick infestations or its anti-haemostatic components exert systemi...

journal_title：Experimental parasitology

authors： Carvalho WA,Maruyama SR,Franzin AM,Abatepaulo AR,Anderson JM,Ferreira BR,Ribeiro JM,Moré DD,Augusto Mendes Maia A,Valenzuela JG,Garcia GR,de Miranda Santos IK

更新日期：2010-04-01 00:00:00

abstract：:Trypanosoma cruzi is the ethiological agent for Chagas disease in Latin America. This study aimed to test the trypanocidal effect of licarin A and burchellin isolated from plants in northeastern Brazil. These neolignans were tested on T. cruzi and on peritoneal macrophages, to evaluate drug toxicity. Epimastigote grow...

journal_title：Experimental parasitology

authors： Cabral MM,Barbosa-Filho JM,Maia GL,Chaves MC,Braga MV,De Souza W,Soares RO

更新日期：2010-03-01 00:00:00

abstract：:Lipophosphoglycan (LPG) is structurally characterized by a series of phosphoglycan repeat units. Cellular LPG, isolated from promastigotes, has a very similar structure to culture supernatant LPG, but differs in the average number of phosphorylated oligosaccharide repeat units and in glycan composition. Comparison of ...

journal_title：Experimental parasitology

authors： Kavoosi G,Ardestani SK,Kariminia A,Alimohammadian MH

更新日期：2010-02-01 00:00:00

abstract：:Leishmania donovani, causative organism for visceral leishmaniasis, is responsible for considerable mortality and morbidity worldwide. Generation of drug-resistant variants continue to challenge the chemotherapy, the mainstay to fight the disease. The aim of current study was proteomic profiling of wild type (Ld-Wt) a...

journal_title：Experimental parasitology

authors： Sharma S,Singh G,Chavan HD,Dey CS

更新日期：2009-12-01 00:00:00

abstract：:A Real-time quantitative PCR assay to quantify the Toxoplasma gondii apicoplast was studied. Primers were designed to amplify a 305bp product specific to T. gondii apicoplast. Standard curves were generated for both T. gondii apicoplast DNA and genomic DNA, and were used to compute the relative concentration of apicop...

journal_title：Experimental parasitology

authors： Wu L,Chen SX,Jiang XG,Cao JP

更新日期：2009-12-01 00:00:00

abstract：:Two recombinant barley cystatins, HvCPI5 and HvCPI6, have been tested in vitro against promastigotes and intracellular amastigotes of Leishmania infantum in the J774 monocytic cell line. Toxicity of cystatins for J774 cells was also determined. In addition, a comparison between direct counts of intracellular amastigot...

journal_title：Experimental parasitology

authors： Ordóñez-Gutiérrez L,Martínez M,Rubio-Somoza I,Díaz I,Mendez S,Alunda JM

更新日期：2009-12-01 00:00:00

abstract：:Acanthamoeba spp., known to cause keratitis and granulomatous encephalitis in humans, are frequently isolated from a variety of water sources. Here we report for the first time the characterization of an Acanthamoeba sp. (ACC01) isolated from tap water in Brazil. This organism is currently being maintained in an axeni...

journal_title：Experimental parasitology

authors： Magliano AC,da Silva FM,Teixeira MM,Alfieri SC

更新日期：2009-11-01 00:00:00

abstract：:In this study, human sera reactivity against nine peptides derived from the Toxoplasma gondii P30 protein was assessed by ELISA in patients with different clinical forms of toxoplasmosis. Same as has been reported in mice, sera from congenital, ocular and chronic asymptomatic toxoplasmosis patients recognized more str...

journal_title：Experimental parasitology

authors： Cardona N,de-la-Torre A,Siachoque H,Patarroyo MA,Gomez-Marin JE

更新日期：2009-10-01 00:00:00

abstract：:In order to examine the role of IFN-gamma, TNFRp55 and iNOS in inflammatory reaction during toxoplasmosis, IFN-gamma(-/-), TNFRp55(-/-) and iNOS(-/-) mice were experimentally infected with Toxoplasma gondii ME-49 strain. The organs of the mice were evaluated for histology and immunohistochemistry in detection of tissu...

journal_title：Experimental parasitology

authors： Silva NM,Vieira JC,Carneiro CM,Tafuri WL

更新日期：2009-09-01 00:00:00

abstract：:Leishmania parasites lack catalase and therefore, their anti-oxidant system hinges primarily upon non-protein thiols; accordingly, depletion of thiols could potentially serve as an effective drug target. We have developed a flow cytometry based assay using 5-chloromethyl fluorescein diacetate based upon its selective ...

journal_title：Experimental parasitology

authors： Sarkar A,Mandal G,Singh N,Sundar S,Chatterjee M

更新日期：2009-08-01 00:00:00

abstract：:Considering that the treatment for toxoplasmosis is based on drugs that show limited efficacy due to their substantial side effects, the purpose of the present study was to evaluate the effects of Artemisia annua on in vitro and in vivo Toxoplasma gondii infection. A. annua infusion was prepared from dried herb and te...

journal_title：Experimental parasitology

authors： de Oliveira TC,Silva DA,Rostkowska C,Béla SR,Ferro EA,Magalhães PM,Mineo JR

更新日期：2009-07-01 00:00:00

abstract：:This paper describes the in vitro multiplication process of Babesia bigemina sporokinetes in a cell line (IDE8) from Ixodes scapularis ticks. The inoculum was obtained from hemolymph of engorged females of Rhipicephalus (Boophilus) microplus ticks naturally infected with B. bigemina. These ticks had been fed on calves...

journal_title：Experimental parasitology

authors： Ribeiro MF,Bastos CV,Vasconcelos MM,Passos LM

更新日期：2009-07-01 00:00:00

abstract：:Plasma butyrylcholinesterase (BChE) hydrolyzes ester-containing compounds such as succinylcholine, as well as acting as a scavenger against neurotoxic organophosphates (OPs). We previously found that Nippostrongylus brasiliensis infection makes rats more susceptible to OP toxicity by decreasing serum paraoxonase-1 (PO...

journal_title：Experimental parasitology

authors： Farid AS,Horii Y

更新日期：2009-06-01 00:00:00

abstract：:The sphingolipids biosynthesis pathway generates bioactive molecules crucial to the regulation of physiological processes. We have recently reported that DAG (diacylglycerol) generated during sphingomyelin synthesis, plays an important role in PKC (protein kinase C) activation, necessary for the transit through the ce...

journal_title：Experimental parasitology

authors： Cerbon J,Olguin T,Alvarez-Grave PR,López-Sánchez RC

更新日期：2009-06-01 00:00:00

abstract：:Toxoplasma gondii, an intracellular parasite, has two distinctive growth stages, namely rapidly growing tachyzoites and slowly growing bradyzoites. Here we report a unique physiological function of the last committed glycolytic enzyme of T. gondii, lactate dehydrogenase (TgLDH), which is present in two isoforms and ex...

journal_title：Experimental parasitology

authors： Liwak U,Ananvoranich S

更新日期：2009-06-01 00:00:00

abstract：:In this paper, the lytic activity of two variants of Serratia marcescens against promastigotes of Leishmania braziliensis was studied. In vitro assays showed that S. marcescens variant SM365 lyses L. braziliensis promastigotes, while the variant DB11 did not. Scanning electron microscopy (SEM) revealed that S. marcesc...

journal_title：Experimental parasitology

authors： Moraes CS,Seabra SH,Albuquerque-Cunha JM,Castro DP,Genta FA,de Souza W,Brazil RP,Garcia ES,Azambuja P

更新日期：2009-06-01 00:00:00

abstract：:A cDNA encoding elastase was isolated from Steinernema carpocapsae by suppression subtractive hybridization and rapid amplification of 5' cDNA ends. The predicted protein contained a 19-aa signal peptide, a 44-aa N-terminal propeptide, and a 264-aa mature protein with a predicted molecular mass of 28,949 Da and a theo...

journal_title：Experimental parasitology

authors： Hao YJ,Montiel R,Nascimento G,Toubarro D,Simoes N

更新日期：2009-05-01 00:00:00

abstract：:A Schistosoma mansoni G-protein coupled receptor (SmGPCR) was previously cloned and shown to be activated by the biogenic amine, histamine. Here we report a first investigation of the receptor's subunit organization, tissue distribution and expression levels in different stages of the parasite. A polyclonal antibody w...

journal_title：Experimental parasitology

authors： El-Shehabi F,Vermeire JJ,Yoshino TP,Ribeiro P

更新日期：2009-05-01 00:00:00

abstract：:With the aim of determining selectivity and the possible target(s) of nitroheteroaryl-1,3,4-thiadiazoles considered as possible leads for the development of anti-leishmanial agents, we studied 5-nitroimidazole, 5-nitrofuran and 5-nitrothiophene analogs of N-substituted-piperazinyl-1,3,4-thiadiazoles. We investigated 2...

journal_title：Experimental parasitology

authors： Poorrajab F,Ardestani SK,Foroumadi A,Emami S,Kariminia A,Behrouzi-Fardmoghadam M,Shafiee A

更新日期：2009-04-01 00:00:00